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JoVE Journal Biology
Lentivirus Produktion
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Lentivirus Produktion

Article DOI: 10.3791/1499-v • 11:43 min • October 2nd, 2009
October 2nd, 2009 •
1, 1
1Diabetes Center, University of California, San Francisco - UCSF

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För att göra lentiviruses, är DNA-vektorer transfekterade i människors 293 celler. Efter skörden och koncentrera supernatanten är virus titer bestäms av fluorescens uttryck med en flödescytometer.

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Mikrobiologi lentivirus RNAi viral titrering transfektion retrovirus flödescytometri dela vektorsystem shRNA.
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