Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Antikropp Profilering av luciferas…
JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology
Antikropp Profilering av luciferas Immunoprecipitation Systems (LIPS)
Regulatory T cells: Therapeutic Potential for Treating Transplant Rejection and Type I Diabetes
Journal (Biology)
Regulatory T cells: Therapeutic Potential for Treating Transplant Rejection and Type I Diabetes
Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D
Journal (Biology)
Methods to Study Epithelial Transport Protein Function and Expression in Native Intestine and Caco-2 Cells Grown in 3D
 
Click here for the English version

Antikropp Profilering av luciferas Immunoprecipitation Systems (LIPS)

Article DOI: 10.3791/1549-v • 12:20 min • October 7th, 2009
October 7th, 2009 •
1, 1, 1, 1
1Neurobiology and Pain Therapeutics Section, National Institute of Dental and Craniofacial Research, National Institutes of Health

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

De tekniska aspekterna av att utföra LIPS (luciferas Immunoprecipitation Systems) beskrivs. Den övergripande strategin innebär att uttrycka chimär gener som kodar för antigener smält till Renilla luciferas (RUC) i däggdjursceller. Rå Ruc-antigen extrakt är då förberedda och utan rening, anställd immunoprecipitation analyser för att kvantifiera antikroppar.

Tags

Immunologi 32 antigen autoantikroppar biomarkörer diagnostik ELISA Proteome
Read Article
Waiting X
Simple Hit Counter