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Journal / Biology / Antilichaam Profiling door Luciferase…
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Antilichaam Profiling door Luciferase Immunoprecipitatie Systems (LIPS)
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Antilichaam Profiling door Luciferase Immunoprecipitatie Systems (LIPS)

Article DOI: 10.3791/1549-v • 12:20 min • October 7th, 2009
October 7th, 2009 •
1, 1, 1, 1
1Neurobiology and Pain Therapeutics Section, National Institute of Dental and Craniofacial Research, National Institutes of Health

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De technische aspecten van het uitvoeren van LIPS (Luciferase Immunoprecipitatie Systems) worden beschreven. De algemene benadering bestaat uiten chimere genen die coderen voor antigenen gefuseerd met Renilla luciferase (RUC) in zoogdiercellen. Ruwe Ruc-antigeen extracten worden dan voorbereid en, zonder zuivering, werkzaam in de immunoprecipitatie testen op antilichamen te kwantificeren.

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Immunologie Antigen Auto-antilichamen Biomarker diagnose ELISA Proteome
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