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Generering av inducerade pluripotenta stamceller genom att programmera humana med Stemgent mänskliga TF lentivirus Set
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Generering av inducerade pluripotenta stamceller genom att programmera humana med Stemgent mänskliga TF lentivirus Set

Article DOI: 10.3791/1553-v • 12:10 min • December 8th, 2009
December 8th, 2009 •
1, 1, 1, 1, 1, 1
1Research and Development, Stemgent

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Vi visar protokollet för generering av inducerade pluripotenta stamceller från mänskliga somatiska celler med lentivirus-medierade leverans av mänskliga faktorer Oct4, Sox2, NANOG och Lin28. Pluripotens bekräftades av morfologi och förekomst av embryonala stamceller (ES) cell-specifika markörer.

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Utvecklingsbiologi IPS omprogrammering lentivirus stamcellsforskning inducerade pluripotenta celler pluripotens fibroblast embryonala stamceller celler ES iPS-celler
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