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Isolation and Derivation of Mouse Embryonic Germinal Cells

Article DOI: 10.3791/1635-v • 14:02 min • October 22nd, 2009
October 22nd, 2009 •
1, 1, 1, 1
1Reproductive Genetics and Stem Cell Laboratory, Department of Biological Sciences, Mississippi State University

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The ability of embryonic germinal cells to differentiate into primordial germinal cells during early development stages is a perfect model to address our hypothesis about cancer and infertility. This protocol shows how to isolate primordial germinal cells from developing gonads in 10.5-11.5 days post coitum mouse embryos.

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Isolation Derivation Mouse Embryonic Germinal Cells Primordial Germinal Cells Differentiation Cancer Infertility Protocol Developing Gonads Mouse Embryos Dissociation Mechanical Disruption Collagenase Mouse Embryo Fibroblast Feeder Layer Mitotically Inactivated Knockout Media Leukemia Inhibitor Factor (LIF) Basic Fibroblast Growth Factor (bFGF) Stem Cell Factor (SCF) PCG Identification Establishment Of Culture Conditions Long Term Cultures Embryonic Phenotype Pluripotent State Genetic Damage Epigenetic Damage Oxidative Stress
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