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JoVE Journal
Biology

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通过双光子荧光恢复漂白后测量扩散系数
 
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通过双光子荧光恢复漂白后测量扩散系数

Article DOI: 10.3791/1636-v 07:00 min February 26th, 2010
February 26th, 2010

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Summary

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在这篇文章中,我们将描述测量扩散系数,使用多光子荧光漂白后恢复的过程。我们将开始通过调整样品沿光路的激光,并确定适当的实验参数,然后继续产生,最后装修荧光恢复曲线。

Tags

细胞生物学,第36期,扩散,荧光漂白后恢复,MP - FRAP,玻璃钢,多光子
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