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Använda Gene Pulser MXcell elektroporation System till transfektera primärceller med hög verkningsgrad
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Använda Gene Pulser MXcell elektroporation System till transfektera primärceller med hög verkningsgrad

Article DOI: 10.3791/1662-v • 12:56 min • January 7th, 2010
January 7th, 2010 •
1, 1, 1
1Gene Expression Division, Bio-Rad Laboratories, Inc.

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Denna procedur visar hur du använder Gene Pulser MXcell elektroporation systemet för att snabbt och enkelt identifiera de bästa elektroporation förutsättningarna för mus embryonala fibroblaster (MEFs) eller andra primära celler. Överväganden för felsökning diskuteras också i den tillhörande video.

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Cellbiologi Primär cell elektroporation MEF Bio-Rad Gene Pulser MXcell transfektion GFP
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