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Journal / Biology / 가역 Biotinylation에 의해 플라즈마 막 단백질 Endocytic 요금…
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가역 Biotinylation에 의해 플라즈마 막 단백질 Endocytic 요금 측정

Article DOI: 10.3791/1669-v • 11:33 min • December 23rd, 2009
December 23rd, 2009 •
1, 1, 1
1University of Massachusetts Medical School

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규제 endocytosis는 막 단백질의 대부분의 세포 표면 발현 수준을 관리합니다. 여기서 우리는 도파민 전송 (DAT), polytopic 멤브레인 단백질의 endocytic 속도를 측정하는 줄이할 수있는, 멤브레인 impermeant biotinylation의 시약을 사용합니다. 방법은 대부분의 플라즈마 막 단백질의 endocytic 속도를 측정하는 직접적인 접근을 용이하게합니다.

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세포 생물학 제 34 세포 생물학 멤브레인 거래 endocytosis biotinylation
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