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初代培養およびコルチのマウス臓器のプラスミドエレクトロ。
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Article
DOI:
10.3791/1685-v
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12:30 min
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February 4th, 2010
初代培養およびコルチのマウス臓器のプラスミドエレクトロ。
February 4th, 2010
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1Department of Otology and Laryngology, Harvard Medical School, 2Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary, 3Department of Communication Sciences and Disorders, Emerson College, 4Program in Speech and Hearing Bioscience and Technology, Division of Health Science and Technology, Harvard
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
この手順では、スパイラル輪部とらせん神経節ニューロンの有無にかかわらず、コルチ器のマウス器官の単離および培養のための方法を説明します。我々はまた、エレクトロポレーションにより、コルチ植の器官における外因性レポーター遺伝子の発現のための方法を示しています。