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Journal / Neuroscience / Cerebral Blutoxygenierung Messung auf…
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JoVE Journal Neuroscience
Cerebral Blutoxygenierung Messung auf Sauerstoff-abhängige Quenching der Phosphoreszenz Based
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Cerebral Blutoxygenierung Messung auf Sauerstoff-abhängige Quenching der Phosphoreszenz Based

Article DOI: 10.3791/1694-v • 08:58 min • May 4th, 2011
May 4th, 2011 •
1, 2, 1, 3, 1, 3, 1, 1, 1,4, 3, 2, 1
1Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, 2Department of Biochemistry and Biophysics, University of Pennsylvania, 3Neuroprotection Research Laboratory, Departments of Radiology and Neurology, Massachusetts General Hospital and Harvard Medical School, 4Departments of Neurosciences and Radiology, University of California

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Wir präsentieren ein experimentelles Verfahren zur Messung der Sauerstoff-Partialdruck (pO2) in zerebralen Gefäßen auf Sauerstoff-abhängigen Löschung der Phosphoreszenz basiert. Tierische Vorbereitung und bildgebenden Verfahren wurden sowohl für großes Sichtfeld CCD-basierten Imaging von pO2 in Ratten und 2-Photonen-Anregung Bildgebung des pO2 bei Mäusen beschrieben.

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Neuroscience Ausgabe 51 Hirn Blut Sauerstoffzufuhr Phosphoreszenz Bildgebung
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