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Journal / Immunology and Infection / Neutrofili Trappole extracellulare: Come generare …
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Immunology and Infection

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JoVE Journal Immunology and Infection
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Neutrofili Trappole extracellulare: Come generare e poterle visualizzare

Article DOI: 10.3791/1724-v • 14:17 min • February 24th, 2010
February 24th, 2010 •
1, 1, 1,2, 1,2, 2
1Core Facility Microscopy, Max Planck Institute for Infection Biology, 2Cellular Microbiology, Max Planck Institute for Infection Biology

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Neutrofili Trappole extracellulare (NET) sono un importante meccanismo immunitario innato per combattere i batteri patogeni, funghi e parassiti. Qui si descrivono i metodi per isolare i granulociti neutrofili dal sangue umano e di attivare per formare NET. Vi presentiamo le tecniche di preparazione di visualizzare reti in microscopia ottica ed elettronica.

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JOVE Immunologia Numero 36 neutrofili granulociti neutrofili Trappola extracellulare NET isolamento immunomarcatura microscopia elettronica
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