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Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

Article DOI: 10.3791/1792-v • 07:56 min • May 2nd, 2010
May 2nd, 2010 •
*1, *1, 1, 1, 1
1Department of Medical Neurobiology, Institute for Medical Research Israel Canada, Hebrew University-Hadassah Medical School
* These authors contributed equally

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This video demonstrates how to visualize axonal pathways of genetically defined groups of neurons in the embryonic chick spinal cord utilizing in ovo electroporation of reporter genes under the control of specific enhancer elements.

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Axonal Pathways Genetically Defined Neurons Chick Neural Tube In Ovo Electroporation Enhancer Elements Reporter Genes Tracking Axonal Projection Spinal Interneurons Germ Line-targeted Reporter Genes Bilateral Labeling Ipsi- And Contralaterally Projecting Axons Unilateral Electroporation Central Nervous System Handling Eggs Injection Tungsten Electrodes Electrical Pulses Pulse Generator Incubator Spinal Cord Preparation Whole Mount Antibody Staining Confocal Microscopy
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