JoVE Journal
Biology
Biology
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Summary
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大鼠MSCs从股骨和胫骨分离,然后通过磁性细胞分选富集。排序细胞被证实为流式细胞仪检测表面标志物表达。这些细胞克隆密度培养,形成单菌落,然后这些菌落克隆气瓶分开。