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JoVE Journal
Biology

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Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay
 

Dissection of Organizer and Animal Pole Explants from Xenopus laevis Embryos and Assembly of a Cell Adhesion Assay

Article DOI: 10.3791/187-v 14:22 min April 29th, 2007
April 29th, 2007

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Summary

This video demonstrates the technique used for preparation of organizer and animal pole explants from Xenopus laevis embryos, including the use of the eyebrow knife - a specialized dissection tool made of one's eyebrow. The protocol for assembling an adhesion assay is also given, which probes for the presence of key adhesion molecules present on the surface organizer or animal pole cells that are critical for proper development.

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Dissection Organizer Animal Pole Explants Xenopus Laevis Embryos Assembly Cell Adhesion Assay
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