Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

N -连接糖链的糖蛋白在哺乳动物细胞中的脉冲大通分析
 
Click here for the English version

N -连接糖链的糖蛋白在哺乳动物细胞中的脉冲大通分析

Article DOI: 10.3791/1899-v 10:18 min April 27th, 2010
April 27th, 2010

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

我们描述了一种通过改变早期的生活后,其在哺乳动物细胞中合成的糖蛋白的N -连接聚糖分析方法。这是通过分析代谢标记聚糖,从糖蛋白和高效液相色谱法检查酶的释放脉冲追逐。

Tags

细胞生物学,第38期,N -连接寡糖,甘露糖的标签,内质网相关降解,calnexin,糖,甘露
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter