Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Pulse-chase analyse van N-gebonden suikerketens…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
Pulse-chase analyse van N-gebonden suikerketens van glycoproteïnen in zoogdiercellen
Optimizing the Rearing Procedure of Germ-Free Wasps
Journal (Biology)
Optimizing the Rearing Procedure of Germ-Free Wasps
Dissection and Mounting of Drosophila Pupal Eye Discs
Journal (Biology)
Dissection and Mounting of Drosophila Pupal Eye Discs
 
Click here for the English version

Pulse-chase analyse van N-gebonden suikerketens van glycoproteïnen in zoogdiercellen

Article DOI: 10.3791/1899-v • 10:18 min • April 27th, 2010
April 27th, 2010 •
1, 1, 1, 1, 1
1Department of Cell Research and Immunology, George Wise Faculty of Life Sciences, Tel Aviv University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

We beschrijven een methode voor de analyse van de verandering van N-gekoppelde glycanen door het vroege leven van glycoproteïnen na hun biosynthese in zoogdiercellen. Dit wordt bereikt door pulse-chase analyse van metabolisch gelabeld glycanen, enzymatische vrijlating uit glycoproteïnen en onderzoek door HPLC.

Tags

Cellular Biology N-gekoppelde oligosaccharide mannose-etikettering endoplasmatisch reticulum verbonden degradatie calnexin glycosylering mannosidase
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter