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Zwei-Photonen-Based Photoaktivierung in Live Zebrafischembryonen

Article DOI: 10.3791/1902-v • 09:11 min • December 24th, 2010
December 24th, 2010 •
*1, *1, 1
1Molecular Cell Biology, Weizmann Institute of Science
* These authors contributed equally

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Mehrphotonenmikroskopie ermöglicht die Steuerung von Photonen niedriger Energie mit tiefen optischen Durchbruch und geringere Phototoxizität. Wir beschreiben den Einsatz dieser Technologie für Live-Cell-Kennzeichnung in Zebrafisch-Embryonen. Dieses Protokoll kann leicht für die Foto-Induktion verschiedener Licht-responsive Moleküle angepasst werden.

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Developmental Biology Ausgabe 46 Multi-Photonen-Mikroskopie Uncaging Optogenetik Danio rerio
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