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Journal / Biology / Eukaryotic Polyribosome Profile Analysis
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Eukaryotic Polyribosome Profile Analysis
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Eukaryotic Polyribosome Profile Analysis

Article DOI: 10.3791/1948-v • 09:17 min • June 15th, 2010
June 15th, 2010 •
1, 1, 1, 1, 1, 1, 1, 1
1Department of Molecular Genetics, Microbiology, and Immunology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School

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This article describes a protocol for the extraction of translating ribosomes from eukaryotic cells. Once extracted, ribosomes are separated into monosomes and polyribosomes by sucrose gradient fractionation to allow different ribosomal populations to be analyzed. As such, this method is the gold standard for examining the regulation of translation.

Tags

Eukaryotic Polyribosome Profile Analysis Protein Synthesis Translation Regulation MRNA/ribosome Complexes Sucrose Density Gradient Polyribosomes Monosomes Translation Initiation Elongation/termination MRNA Cohort Experimental Conditions Ribosome Assembly Small Ribosomal Subunit Large Ribosomal Subunit
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