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Photoconversion renat fluorescerande proteiner och Dual-probe optisk markering i levande celler
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Photoconversion renat fluorescerande proteiner och Dual-probe optisk markering i levande celler

Article DOI: 10.3791/1995-v • 11:22 min • June 26th, 2010
June 26th, 2010 •
1, 1
1Department of Molecular Physiology and Biophysics, Vanderbilt University

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Detta protokoll beskriver en allmän strategi för att utföra photoconversion av fluorescerande proteiner på en konfokala laserskanning mikroskop. Vi beskriver förfaranden för photoconversion av puried protein prover, samt för dual-sond optisk lyfta i levande celler med mOrange2 och Dronpa.

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Cellbiologi mOrange Dronpa photoconversion fotoaktivering oktanol droppe konfokal bildhantering
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