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Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
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Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Article DOI: 10.3791/2010-v • 09:48 min • August 21st, 2010
August 21st, 2010 •
1, 2
1The Healing Foundation Centre, Faculty of Life Sciences, University of Manchester, 2Department of Developmental Biology, Washington University School of Medicine

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Summary
Automatic Translation

This video protocol demonstrates a method for generating transgenic Xenopus laevis by introduction of transgenes into sperm nuclei followed by nuclear transplantation into unfertilized eggs.

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Transgenic Xenopus Laevis Restriction Enzyme Nuclear Transplantation Gene Expression Transgenesis Approach Sperm Nuclei Egg Extract Linearized Plasmid DNA Chromosomal Breaks Recombination Embryonic Cleavage Chimeric Embryos
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