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Neuroscience

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JoVE Journal Neuroscience
Ultra-som guiada microinjeção em parte frontal do cérebro do rato In Utero Em E9.5
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Ultra-som guiada microinjeção em parte frontal do cérebro do rato In Utero Em E9.5

Article DOI: 10.3791/2047-v • 07:48 min • November 13th, 2010
November 13th, 2010 •
1, 1,2
1Institute for Cell Engineering Neuroregeneration and Stem Cell Programs, Johns Hopkins University School of Medicine, 2Departments of Neurology, Neuroscience, and Oncology, Johns Hopkins University School of Medicine

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Cirurgia no útero de sobrevivência em camundongos permite a manipulação molecular de expressão de genes durante o desenvolvimento. Aqui nós descrevemos o uso de alta freqüência ultra-sonografia para guiar a injeção de vetores retrovirais para o cérebro do rato no dia embrionário (E) 9.5.

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Neurociência Edição 45 ultra-sonografia de alta freqüência a cirurgia de sobrevivência de roedores intra-ventricular microinjeção vetores retrovirais a transdução genética
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