Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biology

This content is Free Access.

Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras
 

Dechorionation of Medaka Embryos and Cell Transplantation for the Generation of Chimeras

Article DOI: 10.3791/2055-v 09:03 min December 22nd, 2010
December 22nd, 2010

Chapters

Summary

Due to the hard chorion and soft embryos, manipulation of medaka embryos is more involved than in zebrafish. This video shows step-by-step procedures for how to manipulate medaka embryos, including dechorionation, mounting in agarose for imaging and cell transplantation for the production of chimeras. These procedures are essential to use medaka and zebrafish in a laboratory to take full advantage of their complementary features for the genetic dissection of vertebrate genome functions.

Tags

Dechorionation Medaka Embryos Cell Transplantation Chimeras Genetic Analysis Embryological Analysis Vertebrate Model Organisms Mutant Screens Functional Overlap Novel Phenotypes Genetic Dissection Manipulation Of Embryos Imaging Cell Autonomy Fate Maps Tissue Integration Development Hard Chorion Soft Embryos
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter