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Journal / Biology / Kvantifiering av γH2AX foci i vävnadsprover
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Kvantifiering av γH2AX foci i vävnadsprover
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Kvantifiering av γH2AX foci i vävnadsprover

Article DOI: 10.3791/2063-v • 08:48 min • June 28th, 2010
June 28th, 2010 •
1,2, 1,3, 1,2,3, 1, 2,3, 3,4,5, 1,3
1Epigenomic Medicine, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 2Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute, The Alfred Medical Research and Education Precinct, 3Department of Pathology, The University of Melbourne, 4Department of Allergy and Immunology, Murdoch Children's Research Institute, Royal Children's Hospital, 5Department of Pediatrics, The University of Melbourne

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Kvantifiering av DNA dubbel-strängbrott på grundval av γH2AX foci har blivit ett ovärderligt verktyg, särskilt i strålningsbiologi, för utvärdering av vävnaden strålkänslighet och effekter av strålning ändra föreningar. Här har vi demonstrera användningen av ett immunofluorescens test för kvantifiering av γH2AX foci i vävnadsprover.

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Cellbiologi immunofluorescens DNA dubbel-strängbrott histoner variant H2AX DNA-skada joniserande strålning reaktiva syreradikaler
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