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JoVE Journal Neuroscience
Axoplasma de aislamiento de nervio ciático de rata
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Axoplasma de aislamiento de nervio ciático de rata

Article DOI: 10.3791/2087-v • 05:29 min • September 24th, 2010
September 24th, 2010 •
1, 1, 1
1Department of Biological Chemistry, Weizmann Institute of Science

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Demostramos un protocolo para el aislamiento de axoplasma del nervio ciático de rata adulta basada en la disección de los fascículos nerviosos y la incubación en un medio hipotónico para liberar la mielina y la lisis no axonal estructuras, seguido de la extracción del resto del axón material enriquecido.

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Neurociencia Número 43 axoplasma los nervios el aislamiento el método la rata
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