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Análise do genoma usando chip para identificar alvos isoforma específica Gene
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Análise do genoma usando chip para identificar alvos isoforma específica Gene

Article DOI: 10.3791/2101-v • 11:20 min • July 7th, 2010
July 7th, 2010 •
1, 2, 1, 1, 3, 2, 1
1Department of Biochemistry and Molecular Genetics, University of Illinois Chicago - UIC, 2Research Unit on Biomedical Informatics, Universitat Pompeu Fabra, 3Genome Technology Core, Whitehead Institute for Biomedical Research

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Aqui estamos apresentando uma imunoprecipitação da cromatina (CHIP) procedimento para análise do genoma localização das isoformas de proteínas que diferem em um domínio de ligação de histonas. Estamos aplicando-a-CHIP Seq análise para identificar os alvos da demetilase KDM5A/JARID1A/RBP2 histonas.

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Bioquímica Edição 41 cromatina imunoprecipitação ChIP-Seq RBP2 JARID1A KDM5A isoforma específica de recrutamento
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