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Single Cell Transfectie in kippenembryo's
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Single Cell Transfectie in kippenembryo's

Article DOI: 10.3791/2133-v • 08:47 min • September 25th, 2010
September 25th, 2010 •
1, 1
1Department of Medical Neurobiology, Hadassah Medical School - Hebrew University

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Met behulp van fijne punt micropipetten we plasmide DNA injecteren in deelgebieden van de kip somieten of neurale buizen. De concentratie van het plasmide is aangepast aan enkele getransfecteerde cellen te genereren. Vervolgens hebben we laten de cellen zich ontwikkelen tot klonale populaties.

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Developmental Biology single-cell transfectie elektroporatie gen miss-expressie lineage tracing aviaire embryo micro-injectie somiet neurale buis
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