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Journal / Neuroscience / 에듀 신호 증폭 개별 세포 Mitochondrial DNA 복제의…
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Neuroscience

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JoVE Journal Neuroscience
에듀 신호 증폭 개별 세포 Mitochondrial DNA 복제의 시각화
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에듀 신호 증폭 개별 세포 Mitochondrial DNA 복제의 시각화

Article DOI: 10.3791/2147-v • 09:37 min • November 15th, 2010
November 15th, 2010 •
1, 2, 3
1Michigan Research Community, Undergraduate Research Opportunity Program, University of Michigan, 2Department of Neurology, University of Michigan, 3Department of Internal Medicine, Division of Metabolism, Endocrinology, and Diabetes, University of Michigan

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우리는 mtDNA의 biogenesis를 공부하기 위해서는 각각의 세포에서 새로 합성된 DNA mitochondrial (mtDNA)를 라벨에 민감한 기술을 개발했습니다. 기술은 뉴런의 subcellular 구획 내에 mtDNA 복제를 시각화하는 tyramide 신호 증폭 (TSA) 프로토콜과 함께 에듀의 결합이 결합되어 있습니다.

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신경 과학 제 45 mitochondria mitochondrial DNA (mtDNA) 5 - ethynyl - 2' - deoxyuridine (이두) 라벨 tyramide 신호 증폭 mtDNA biogenesis 지느러미 루트 신경절의 뉴런
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