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Journal / Biology / 蓝原生聚丙烯酰胺凝胶电泳（BN -…
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蓝原生聚丙烯酰胺凝胶电泳（BN - PAGE）分析，从细胞裂解液多蛋白复合物
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蓝原生聚丙烯酰胺凝胶电泳（BN - PAGE）分析，从细胞裂解液多蛋白复合物

Article DOI: 10.3791/2164-v • 12:04 min • February 24th, 2011
February 24th, 2011 •
1,2,3, *2,3, *3
1Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, 2Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology, University of Freiburg, 3Department of Molecular Immunology, Max-Planck-Institute of Immunology and Epigenetics
* These authors contributed equally

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在这个视频中，我们描述的多蛋白复合物（储值卡）蓝色原生聚丙烯酰胺凝胶电泳（BN - PAGE）的表征。在第一个层面，透析细胞裂解液的BN - PAGE分离，以确定个人的多用途储值卡。在第二维SDS - PAGE电泳，利益的储值卡是进一步细分免疫印迹分析其成分。

Tags

生物化学，第48期，BN - PAGE，2D凝胶电泳，细胞裂解液，透析，蛋白复合物，多蛋白复合物，蛋白质相互作用
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