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Journal / Biology / Hücresel lizatları Multiprotein Kompleksleri…
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Hücresel lizatları Multiprotein Kompleksleri Analizi Mavi Native Poliakrilamid Jel Elektroforez (BN-PAGE)
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Hücresel lizatları Multiprotein Kompleksleri Analizi Mavi Native Poliakrilamid Jel Elektroforez (BN-PAGE)

Article DOI: 10.3791/2164-v • 12:04 min • February 24th, 2011
February 24th, 2011 •
1,2,3, *2,3, *3
1Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, 2Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology, University of Freiburg, 3Department of Molecular Immunology, Max-Planck-Institute of Immunology and Epigenetics
* These authors contributed equally

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Bu video, mavi yerli poliakrilamid jel elektroforezi (BN-PAGE) multiprotein kompleksler (MPCs) karakterizasyonu açıklar. Birinci boyut, diyaliz hücresel lizatları bireysel MPCs tanımlamak için BN-PAGE ile ayrılır. Ikinci bir boyut SDS-PAGE, faiz MPCs daha immün bileşenleri analiz alt gruba ayrılır.

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Biyokimya Sayı 48 BN-PAGE 2D jel elektroforezi hücresel lizat diyaliz protein kompleksi multiprotein kompleks protein etkileşim
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