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Immunofluorescentie detectie van twee thymidine-analogen (CldU en IDU) in het jeugdwerk Tissue
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Immunofluorescentie detectie van twee thymidine-analogen (CldU en IDU) in het jeugdwerk Tissue

Article DOI: 10.3791/2166-v • 10:55 min • December 7th, 2010
December 7th, 2010 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Division of Endocrinology and Diabetes, Children’s Hospital of Philadelphia, Institute of Diabetes Obesity and Metabolism, Institute for Regenerative Medicine, Department of Pediatrics, University of Pennsylvania-School of Medicine
* These authors contributed equally

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We hebben afgeleid van een strategie om sequentiële opname van thymidine-analogen (CldU en IDU) op te sporen in de weefsels van volwassen muizen om twee opeenvolgende rondes van celdeling te kwantificeren. Deze strategie is nuttig om de celvernieuwing van de langlevende weefsels, oncogene transformatie, of transit-amplificerende cellen te detecteren.

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Immunologie CldU IDU analoog van thymidine immunofluorescentie Lineage-Tracing Muis celdeling replicatie omzet Stem Cell
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