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Immunfluoreszenz-Nachweis von zwei Thymidinanaloga (CldU und IDU) in primärem Gewebe
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Immunfluoreszenz-Nachweis von zwei Thymidinanaloga (CldU und IDU) in primärem Gewebe

Article DOI: 10.3791/2166-v • 10:55 min • December 7th, 2010
December 7th, 2010 •
*1, *1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Division of Endocrinology and Diabetes, Children’s Hospital of Philadelphia, Institute of Diabetes Obesity and Metabolism, Institute for Regenerative Medicine, Department of Pediatrics, University of Pennsylvania-School of Medicine
* These authors contributed equally

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Wir haben eine Strategie zur sequentiellen Aufnahme von Thymidin-Analoga (CldU und IDU) in das Gewebe erwachsener Mäuse zu erkennen an zwei aufeinander folgenden Runden der Zellteilung zu quantifizieren abgeleitet. Diese Strategie ist sinnvoll, die Zellerneuerung an langlebigen Geweben, onkogenen Transformation oder die Durchreise verstärkenden Zellen zu erkennen.

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Immunologie CldU IDU Thymidinanalogon Immunfluoreszenz Lineage-Tracing Mouse Cell Division Replication Umsatz Stem Cell
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