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Isolation of Stem Cells from Human Pancreatic Cancer Xenografts

Article DOI: 10.3791/2169-v • 11:45 min • September 26th, 2010
September 26th, 2010 •
1, 1, 1
1Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine

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Summary
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Cancer stem cells (CSCs) have been identified in a number of malignancies. In this protocol we describe a flow cytometric method utilizing aldehyde dehydrogenase activity and CD44 and CD24 expression to isolate CSCs from human pancreatic adenocarcinoma xenografts. These viable cells can then be used in functional and analytical studies.

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Isolation Stem Cells Human Pancreatic Cancer Xenografts CSCs Self-renewal Differentiated Progeny Tumor Immunocompromised Mice Breast Cancer Cell Surface Antigen Expression CD44+CD24low/- ALDH Activity Malignant Tissues Pancreatic Adenocarcinoma CD133 Tumorigenic Populations Invasive Cells Protocol Viable Pancreatic CSCs Low-passage Human Xenografts
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