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Immunology and Infection

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Formadoras de Colônias celular Assay (CFC) para células hematopoiéticas humanas
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Formadoras de Colônias celular Assay (CFC) para células hematopoiéticas humanas

Article DOI: 10.3791/2195-v • 11:30 min • December 18th, 2010
December 18th, 2010 •
1, 1, 1
1Department of Pathology and Immunology, Washington University School of Medicine

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O ensaio de células formadoras de colônia (CFC) é um ensaio in vitro em que células progenitoras hematopoéticas formar colônias em meio semi-sólido. Uma combinação de morfologia da colônia, morfologia celular e citometria de fluxo são utilizados para avaliar a capacidade dos progenitores a proliferar e se diferenciar ao longo dos diferentes linhagens hematopoiéticas.

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Emissão de medicina 46 CFC ensaio células progenitoras hematopoéticas CD34 metilcelulose citometria de fluxo Wright / Giemsa
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