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Journal / Biology / Protokoll för RNAi-tester på vuxna myggor (A.…
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Protokoll för RNAi-tester på vuxna myggor (A. gambiae)
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Protokoll för RNAi-tester på vuxna myggor (A. gambiae)

Article DOI: 10.3791/230-v • 11:49 min • July 4th, 2007
July 4th, 2007 •
1, 1
1Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University

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Omvänd genetiska metoder har visat sig extremt användbart för att avgöra vilka gener som underliggande motstånd mot vektor patogener i myggor. Denna video protokoll illustrerar en metod som Dimopoulos labbet att injicera dsRNA i Anopheles gambiae myggor som hyser malariaparasiten. Tekniken manipulera injektionen setup och injicera dsRNA i bröstkorgen illustreras.

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Cellbiologi mygga malaria genetik injektion RNAi denguefeber transgena reproduktionsnivån Genetisk Drive
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