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Journal / Biology / 使用胚体冰冻切片分析的多能干细胞
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使用胚体冰冻切片分析的多能干细胞
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使用胚体冰冻切片分析的多能干细胞

Article DOI: 10.3791/2344-v • 06:55 min • December 8th, 2010
December 8th, 2010 •
*1, *1, *1, 1, 1
1Laboratório Nacional de Céulas-Tronco Embrionárias (LaNCE), Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro (UFRJ), Brazil
* These authors contributed equally

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多能干细胞悬浮液中生长分化成胚状体（EBS）。在这里，我们展示了如何获得高品质的EB冰冻切片，用于研究胚胎发育的细胞和分子方面，同时保留其作为集合体的组织。

Tags

发育生物学，第46期，胚胎干细胞，胚体，冰冻切片，immunochytochemistry，H9
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