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In vivo et in vitro de l'adaptateur-clathrine Interaction
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In vivo et in vitro de l'adaptateur-clathrine Interaction

Article DOI: 10.3791/2352-v • 17:14 min • January 26th, 2011
January 26th, 2011 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, Colorado State University

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Clathrine endocytose dépend de protéines adaptatrices qui coordonnent la sélection de fret et de l'assemblage manteau de clathrine. Nous décrivons ici les procédures pour étudier l'adaptateur clathrine interaction physique et imagerie des cellules vivantes approches utilisant comme modèle la levure Sla1p endocytose protéine adaptatrice.

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Biologie cellulaire numéro 47 la clathrine adaptateur Sla1p déroulez immunoprécipitation la GFP microscopie à fluorescence
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