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In vivo e in vitro de Estudos Adaptor-clathrin Interação
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In vivo e in vitro de Estudos Adaptor-clathrin Interação

Article DOI: 10.3791/2352-v • 17:14 min • January 26th, 2011
January 26th, 2011 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, Colorado State University

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Clatrina-endocitose mediada depende de proteínas adaptadoras que coordenam a seleção de carga e montagem casaco de clatrina. Aqui nós descrevemos os procedimentos para estudar adaptador clathrin interação física e imagens de células vivas abordagens utilizando como modelo a levedura Sla1p proteína endocítica adaptador.

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Biologia Celular Edição 47 adaptador clatrina Sla1p pull down imunoprecipitação GFP microscopia de fluorescência
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