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Rapido PCR termici utilizzando microscala convezione termica
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Rapido PCR termici utilizzando microscala convezione termica

Article DOI: 10.3791/2366-v • 09:02 min • March 5th, 2011
March 5th, 2011 •
1, 2, 3
1Department of Mechanical Engineering, Texas A&M University, 2Department of Mechanical Engineering and Department of Nuclear Engineering, Texas A&M University, 3Department of Chemical Engineering, Texas A&M University

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Descriviamo un nuovo metodo per eseguire la replicazione del DNA tramite la reazione a catena della polimerasi (PCR). Convezione termica viene sfruttata per reagenti navetta continuo tra denaturazione, ricottura, e le condizioni di estensione, mantenendo opposte superfici del reattore a temperatura costante. Questo design intrinsecamente semplice promette di rendere rapido PCR più accessibile.

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Biologia Molecolare Numero 49 reazione a catena della polimerasi PCR il DNA convezione termica
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