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JoVE Journal
Neuroscience

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Nucleofection和胚胎小鼠海马和皮层神经元的原代培养
 
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Nucleofection和胚胎小鼠海马和皮层神经元的原代培养

Article DOI: 10.3791/2373-v 15:40 min January 24th, 2011
January 24th, 2011

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Summary

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Click here for the English version.

该协议概述解剖所需的步骤,通过电穿孔和文化小鼠海马和皮层神经元的转染。短期可用于文化研究轴突的生长和指导,而长期的文化可以为研究突触和树突棘分析。

Tags

神经科学杂志,47期,海马,皮质,转,nucleofection,电,小学文化
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