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Rotulagem F-actina Termina farpado com Rodamina-actina em permeabilizadas Cones de Crescimento Neuronal
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Rotulagem F-actina Termina farpado com Rodamina-actina em permeabilizadas Cones de Crescimento Neuronal

Article DOI: 10.3791/2409-v • 09:15 min • March 17th, 2011
March 17th, 2011 •
1, 1
1Department of Neuroscience, University of Minnesota

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Um método de visualizar e quantificar F-actina termina farpado em cones de crescimento neuronal é descrito. Depois de neurônios cultura em lamínulas, as células são permeabilizadas com uma solução contendo saponina. Em seguida, um de incubação curto com o buffer contendo saponina rodamina-actina incorpora actina fluorescente para fins de actina livre farpado.

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Neurociência Edição 49 Actina cones de crescimento termina farpado polimerização pistas de orientação
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