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Heterokaryon Techniek voor de analyse van celtype-specifieke lokalisatie
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Heterokaryon Techniek voor de analyse van celtype-specifieke lokalisatie

Article DOI: 10.3791/2488-v • 09:31 min • March 11th, 2011
March 11th, 2011 •
*1, *1, 1
1Department of Chemistry and Biochemistry, Worcester Polytechnic Institute- WPI
* These authors contributed equally

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Een flexibele en efficiënte methode voor de karakterisering van celtype-specifieke eiwit lokalisatie en nucleocytoplasmic pendelen wordt beschreven. Deze heterokaryon aanpak maakt gebruik van fluorescent gelabelde fusie-eiwitten om de afbeelding eiwit lokalisaties na celfusie. Het protocol vatbaar is voor steady-state lokalisaties of meer dynamische vaststellingen op basis van live-cell imaging.

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Cellular Biology Heterokaryon fluorescentie microscopie lokalisatie celfusie nucleocytoplasmic pendelen
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