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Journal / Biology / Heterokaryon Teknik för analys av Cell…
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Heterokaryon Teknik för analys av Cell Typspecifika Localization
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Heterokaryon Teknik för analys av Cell Typspecifika Localization

Article DOI: 10.3791/2488-v • 09:31 min • March 11th, 2011
March 11th, 2011 •
*1, *1, 1
1Department of Chemistry and Biochemistry, Worcester Polytechnic Institute- WPI
* These authors contributed equally

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En flexibel och effektiv metod för karakterisering av celltyp specifika proteiner lokalisering och nucleocytoplasmic skytteltrafik beskrivs. Denna heterokaryon tillvägagångssätt använder fluorescerande-märkta fusionsproteiner till lokaliseringar bilden protein efter cellfusion. Protokollet är mottaglig för steady-state lokaliseringar eller mer dynamiskt bestämningar baserade på levande cell imaging.

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Cellbiologi Heterokaryon fluorescensmikroskopi lokalisering cellfusion nucleocytoplasmic skytteltrafik
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