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Die Kristallisation von Membranproteinen in Lipidische Mesophasen
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Die Kristallisation von Membranproteinen in Lipidische Mesophasen

Article DOI: 10.3791/2501-v • 11:53 min • March 28th, 2011
March 28th, 2011 •
1, 1
1Molecular Biology, The Scripps Research Institute

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Die Protokolle beschreiben die wesentlichen Schritte für den Erhalt Beugung Qualität Kristalle von Membranproteinen ab Rekonstitution des Proteins in einer Lipid-kubische Phase (LCP), finden Anfangsbedingungen mit LCP-FRAP Vorkristallisation Assays, die Einrichtung von LCP Kristallisation Studien und Ernte-Kristallen .

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Structural Biology Ausgabe 49 Membran-Protein Lipid-kubische Phase Kristallisation Fluorescence Recovery After Photobleaching (FRAP) G-Protein gekoppelten Rezeptoren
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