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Isolamento de rato células epiteliais respiratórias e exposição à fumaça de cigarro Experimental da Air interface líquido
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Isolamento de rato células epiteliais respiratórias e exposição à fumaça de cigarro Experimental da Air interface líquido

Article DOI: 10.3791/2513-v • 10:48 min • February 21st, 2011
February 21st, 2011 •
1,2, 1, 1
1Department of Medicine, Pulmonary and Critical Care Medicine, Brigham and Women’s Hospital, Harvard Medical School, 2Cellular and Molecular Pathology, School of Medicine, University of Pittsburgh

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Células epiteliais pulmonares podem ser isolados do trato respiratório de camundongos e cultivadas em ar-líquido interface como um modelo de epitélio respiratório diferenciado. Um protocolo é descrito para o isolamento, cultura e expondo essas células à fumaça de cigarro mainstream, a fim de estudar as respostas moleculares para esta toxina ambiental.

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Emissão de medicina 48 ar-líquido Interface isolamento Cell fumaça do cigarro as células epiteliais
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