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Journal / Biology / Arabidopsis thaliana Polar Glycerolipid…
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Arabidopsis thaliana Polar Glycerolipid Profilering med tunnskiktskromatografi (TLC) i kombination med vätske-gaskromatografi (GLC)
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Arabidopsis thaliana Polar Glycerolipid Profilering med tunnskiktskromatografi (TLC) i kombination med vätske-gaskromatografi (GLC)

Article DOI: 10.3791/2518-v • 13:03 min • March 18th, 2011
March 18th, 2011 •
1, 1
1Department of Biochemistry and Molecular Biology, Michigan State University

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Sammansättning av polära lipider extrakt och fettsyresammansättningen enskilda glycerolipids avgörs i en enkel och robust lipid profilering experiment. För detta ändamål är glycerolipids isoleras genom tunnskiktskromatografi och utsattes för transmethylation sina acyl grupper. Fet acyl methylesters kvantifieras genom gas-vätskekromatografi.

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Växtbiologi Lipid Analys Galactolipids Tunnskiktsplattor Chromatogrpahy lipider Chlorplast Arabidopsis
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