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Journal / Bioengineering / Haute profilage de microARN Débit: Multiplex…
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Bioengineering

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JoVE Journal Bioengineering
Haute profilage de microARN Débit
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Haute profilage de microARN Débit: Multiplex Optimisé qRT-PCR à l'échelle nanolitres sur l'IFC de Fluidigm ArrayTM dynamique

Article DOI: 10.3791/2552-v • 07:27 min • August 3rd, 2011
August 3rd, 2011 •
1,2,3, 1,2,4, 5, 1,2,6, 1,2,3,7
1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, 2Center for Reproductive Sciences, University of California San Francisco, 3Department of Urology, University of California San Francisco, 4Department of Cell and Tissue Biology, University of California San Francisco, 5Fluidigm Corporation, Fluidigm Corporation, 6Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 7UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco

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Nous décrivons ici une inversion optimisé multiplexe transcriptase PCR quantitative (qRT-PCR) en combinaison avec le protocole d'une plateforme microfluidique comme un coût et le temps moyen efficace de dépistage à haut débit pour microARN (miARN) les niveaux d'expression, surtout quand on travaille avec des quantités limitées de l'échantillon.

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Bioingénierie Numéro 54 microARN multiplex qRT-PCR le profilage à haut débit Fluidigm
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