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Journal / Bioengineering / High Throughput MicroRNA Profiling:…
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Bioengineering

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JoVE Journal Bioengineering
High Throughput MicroRNA Profiling
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High Throughput MicroRNA Profiling: Geoptimaliseerd Multiplex qRT-PCR op nanoliter Schaalverdeling op de Fluidigm Dynamic ArrayTM IFCS

Article DOI: 10.3791/2552-v • 07:27 min • August 3rd, 2011
August 3rd, 2011 •
1,2,3, 1,2,4, 5, 1,2,6, 1,2,3,7
1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, 2Center for Reproductive Sciences, University of California San Francisco, 3Department of Urology, University of California San Francisco, 4Department of Cell and Tissue Biology, University of California San Francisco, 5Fluidigm Corporation, Fluidigm Corporation, 6Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 7UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco

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Hier beschrijven we een geoptimaliseerde multiplex reverse transcriptase kwantitatieve PCR (qRT-PCR)-protocol in combinatie met een microfluïdisch platform als een kost en tijd efficiënte high-throughput screening tool voor microRNA (miRNA) expressie niveaus, in het bijzonder bij het werken met beperkte hoeveelheden van het monster.

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Bioengineering microRNA multiplex-PCR qRT high throughput profilering Fluidigm
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