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Journal / Bioengineering / Profiling MicroRNA de Alto Rendimento: Multiplex…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Profiling MicroRNA de Alto Rendimento
Tissue Engineering of the Intestine in a Murine Model
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Tissue Engineering of the Intestine in a Murine Model
Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
Journal (Bioengineering)
Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
 
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Profiling MicroRNA de Alto Rendimento: Multiplex Optimized qRT-PCR em Escala nanolitros no IFCS Fluidigm dinâmico ArrayTM

Article DOI: 10.3791/2552-v • 07:27 min • August 3rd, 2011
August 3rd, 2011 •
1,2,3, 1,2,4, 5, 1,2,6, 1,2,3,7
1The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, University of California San Francisco, 2Center for Reproductive Sciences, University of California San Francisco, 3Department of Urology, University of California San Francisco, 4Department of Cell and Tissue Biology, University of California San Francisco, 5Fluidigm Corporation, Fluidigm Corporation, 6Department of Obstetrics and Gynecology, Hadassah-Hebrew University Medical Center, 7UCSF - Helen Diller Family Comprehensive Cancer Center, University of California San Francisco

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Aqui nós descrevemos um otimizado multiplex da transcriptase reversa PCR quantitativo protocolo (qRT-PCR) em combinação com uma plataforma microfluídica como ferramenta de triagem de custo e tempo efetivo de alto rendimento para microRNA (miRNA) níveis de expressão, especialmente quando se trabalha com quantidades limitadas de amostra.

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Emissão de bioengenharia 54 microRNA multiplex qRT-PCR perfil de alto rendimento Fluidigm
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