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JoVE Journal
Biology

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从小鼠胚胎肾肾区细胞的分离和培养
 
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从小鼠胚胎肾肾区细胞的分离和培养

Article DOI: 10.3791/2555-v 08:07 min April 22nd, 2011
April 22nd, 2011

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

在这份报告中,我们描述了一种从小鼠胚胎肾,可用于研究规范发展肾干/祖细胞的信号转导通路的祖细胞小生境的隔离和文化的方法。这些培养的​​细胞是高度开放的小分子和重组蛋白治疗,更重要的还的病毒转导,这使得候选人途径的有效操纵。

Tags

细胞生物学,50期,肾脏发育,nephrogenesis,肾区,肾祖细胞,皮质基质
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