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Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method

Article DOI: 10.3791/2574-v • 12:10 min • March 28th, 2011
March 28th, 2011 •
1, 1
1Molecular Biophysics and Biochemistry, Yale University

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This article describes a standard method to get a three-dimensional (3D) reconstruction of biological macromolecules using negative staining electron microscopy (EM). In this protocol, we explain how to get the 3D structure of the Saccharomyces cerevisiae exosome complex at medium resolution using the random conical tilt reconstruction method (RCT).

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Single Particle Electron Microscopy EM Reconstruction Exosome Complex Random Conical Tilt Method X-ray Crystallography Macromolecular Complexes Protein Sample Negative Staining EM Method Resolution Symmetry Heavy Metal Solution Transmission Electron Microscope 2D Micrographs Protein Molecules
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